Fig. 7

Effect of simultaneous administration of alcohol with THC on pial arteriole diameter under different anesthetics in female rats. A Averaged fold-change in female pial arteriole diameter as a function of time following carotid artery infusion of 0.9% NaCl (saline) under KX anesthesia (n = 4) or 50 mM EtOH with 42 nM THC under KX (n = 5). Asterisks (*) reflect statistically significant difference between saline and 50 mM EtOH with 42 nM THC groups with 0.021 ≤ p ≤ 0.0317 by 1-tail Mann–Whitney test. tr: statistical trend with 0.05 ≤ p ≤ 0.10 by 1-tail Mann–Whitney test. B Averaged fold-change in female pial arteriole diameter as a function of time following carotid artery infusion of 0.9% NaCl under isoflurane (n = 4), and 50 mM EtOH with 42 nM THC under isoflurane (n = 4). Asterisk depicts statistically significant differences with p = 0.0285 by 1-tail Mann–Whitney test. tr: statistical trend with 0.05 ≤ p ≤ 0.10 by 1-tail Mann–Whitney test. C Scattered graph showing effect of simultaneous application of 50 mM EtOH with 42 nM THC on male pial cerebral arteriole diameter in female rats under ketamine versus isoflurane anesthesia. Each datapoint within each group was normalized to averaged effect of 0.9% NaCl at the same time-point within respective group. Asterisks (*) reflect statistically significant difference between isoflurane and ketamine groups with 0.021 ≤ p ≤ 0.0317 by 1-tail Mann–Whitney test. tr: statistical trend with 0.008 ≤ p ≤ 0.0365 by 1-tail Mann–Whitney test