Fig. 6

Anesthetics largely fail to alter effect of simultaneous application of alcohol with THC on cerebral arteriole diameter in male rats. A Averaged fold-change in male pial arteriole diameter as a function of time following carotid artery infusion of 0.9% NaCl (saline) under KX anesthesia (n = 8) or 50 mM EtOH with 42 nM THC under KX (n = 11). Asterisks (*) reflect statistically significant difference between saline and 50 mM EtOH with 42 nM THC groups with 0.025 ≤ p ≤ 0.031 by 1-tail Mann–Whitney test. B Averaged fold-change in male pial arteriole diameter as a function of time following carotid artery infusion of 0.9% NaCl under isoflurane (n = 6), and 50 mM EtOH with 42 nM THC under isoflurane (n = 6). Asterisks (*) reflect statistically significant difference between saline and 50 mM EtOH with 42 nM THC groups with 0.013 ≤ p ≤ 0.0465 by 1-tail Mann–Whitney test. tr: statistical trend with 0.05 ≤ p ≤ 0.10 by 1-tail Mann–Whitney test. C Scattered graph showing effect of simultaneous application of 50 mM EtOH with 42 nM THC on male pial cerebral arteriole diameter in male rats under ketamine versus isoflurane anesthesia. Each datapoint within each group was normalized to averaged effect of 0.9% NaCl at the same time-point within respective group. Asterisk depicts statistically significant differences with p = 0.0145 by 1-tail Mann–Whitney test