Fig. 4

THC effect on pial arteriole diameter in male rats is influenced by anesthetics. A Averaged fold-change in male pial arteriole diameter as a function of time following carotid artery infusion of DMSO-containing saline under KX anesthesia (n = 4) or 42 nM THC under KX (n = 7). B Averaged fold-change in male pial arteriole diameter as a function of time following carotid artery infusion of DMSO-containing saline under isoflurane (n = 5), and 42 nM THC under isoflurane (n = 6). Asterisk depicts statistically significant difference in isoflurane group with p = 0.0026 by 1-tail Mann–Whitney test. C Scattered graph comparing THC effect on male pial cerebral arteriole diameter under KX versus isoflurane anesthesia. Each datapoint within each group was normalized to averaged effect of DMSO-containing saline at the same time-point within respective group. Asterisks depict statistically significant differences with p = 0.0364 by 1-tail Mann–Whitney test. tr: statistical trend with 0.05 ≤ p ≤ 0.10 by 1-tail Mann–Whitney test