Fig. 2

Alcohol effect on pial arteriole diameter under different anesthetics in male rats. A Averaged fold-change in male pial arteriole diameter as a function of time following carotid artery infusion of 0.9% NaCl (saline) under KX anesthesia (n = 8) or 50 mM EtOH under KX (n = 10). Asterisks (*) reflect statistically significant difference between saline and 50 mM EtOH groups with 0.0119 ≤ p ≤ 0.046 by 1-tail Mann–Whitney test. For each group here and in Figs. 6B, 7A-B, 2A-B, 3A-B, 4A-B, and 5A-B, data from individual rats were normalized to their respective arteriole diameter at the beginning of diameter monitoring. For visual clarity, in all aforementioned panels, data are presented as mean ± S.E. Here and in B, inserts are of representative cranial window images from male rats showing vasoconstriction by 50 mM EtOH under KX versus isoflurane anesthesia. B Averaged fold-change in male pial arteriole diameter as a function of time following carotid artery infusion of 0.9% NaCl under isoflurane (n = 6), and 50 mM EtOH under isoflurane (n = 6). Asterisk reflects statistically significant difference with p = 0.02 by 1-tail Mann–Whitney test. tr: statistical trend with 0.05 ≤ p ≤ 0.10 by 1-tail Mann–Whitney test. C Scattered graph showing EtOH effect on male pial cerebral arteriole diameter in male rats under ketamine versus isoflurane anesthesia. Each datapoint within each group was normalized to averaged effect of 0.9% NaCl at the same time-point within respective group. Asterisks depict statistically significant differences with 0.0179 ≤ p ≤ 0.0411 by 1-tail Mann–Whitney test