Fig. 5

The effects of HIF-1α inhibitors on SMIR-induced endothelial permeability, NF200, AQP1 and pain sensitivity. SMIR induced high permeability and axonal plasticity of BNB through HIF-1α/AQP1, and significantly increased pain sensitivity. (A) Evans blue exudation was significantly decreased at 60 min and at 120 min after injection (*P < 0.05, **P < 0.01, vs. the 3rd day post-surgery group. ^##P < 0.01, ^###P < 0.001 vs. SMIR + DMSO group; n = 5). (B) PWMT was significantly increased at 60 min (*P < 0.05 vs. SMIR + DMSO group) and 120 min (***P < 0.001 vs. SMIR + DMSO group) after injection in SMIR group; n = 5. (C) HIF-1α levels were significantly decreased after injection (*P < 0.05, ***P < 0.001 vs. the 3rd day post-surgery group. ^#P < 0.05, ^###P < 0.001 vs. SMIR + DMSO group; n = 5). (D) AQP1 levels were significantly increased at 60 and 120 min after injection (*P < 0.05, **P < 0.01 vs. the 3rd day post-surgery group. ^#P < 0.05, ^##P < 0.01 vs. SMIR + DMSO group; n = 5). (E) NF200 was significantly decreased at 120 min after injection (****P < 0.0001 vs. the 3rd day post-surgery group. ^##P < 0.01 vs. SMIR + DMSO group; n = 5)