Fig. 4

Localization of AQP1 in subneuronal cells in DRG post-SMIR. Immunofluorescence staining showed the localization changes of AQP1 in neurons before and after surgery. (A) Naive group, AQP1 and peptide neuronal marker CGRP had no obvious co-labeling in Naive group. (B) Co-labeling of AQP1 and peptide neuronal marker CGRP increased 3d after surgery. (C) At 7 days after surgery, there was a small amount of co-labeling between AQP1 and the peptide neuronal marker CGRP. (D)There was no obvious co-labeling between AQP1 and peptide neuronal marker CGRP at 14 days after surgery. (E-H) AQP1 and large neuron marker NF200 (co-labeling by immunization) were shown before surgery and at 3, 7, and 14 days after surgery, respectively: (F) AQP1 and large neuron marker NF200 were significantly co-labeling at 3 days after surgery; (E, G, H): no obvious co-standard