Fig. 2

Effects of SMIR on BNB, AQP1 and HIF-1α levels in DRG. (A) SMIR induced BNB dysfunction in DRG and promoted microvascular endothelial hyperpermeability. The amount of Evans blue exudation was significantly increased on the 3rd and 7th day post-surgery in the SMIR group (**P < 0.01, ***P < 0.001 vs. naive group. ^##P < 0.01, ^###P < 0.001 vs. sham group; n = 5). (B-C)Changes of AQP1 and HIF-1α protein expression in DRG after SMIR. SMIR promoted HIF-1α expression and inhibited AQP1 expression in DRG. On the 3rd and 7th days post-surgery, there was a marked decrease in AQP1 levels. HIF-1α protein expression was significantly elevated in the SMIR group on the 3rd day post-surgery (*P < 0.01 vs. naive group; n = 5)