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Fig. 4 | BMC Anesthesiology

Fig. 4

From: Selective optogenetic modulation of the PBN terminals in the lateral hypothalamic area and basal forebrain regulates emergence from isoflurane anesthesia in mice

Fig. 4

Optical inhibition of PBN-BF projections delays emergence from isoflurane anesthesia. A Schematic of inhibitory optogenetic virus (NpHR) injection into the PBN and optical implant into the BF in mice. B Histological and fluorescence image showing the virus expression in the PBN and BF. C Schematic showing the protocol of optogenetic suppression of PBN-BF projections during isoflurane anesthesia. D and E LORR time and RORR time of mice with optogenetic inhibition of PBN-BF projections, n = 8 per group. F Representative EEG traces (above) and corresponding power spectra (below) optical activation under 1.5% isoflurane anesthesia in the EYEP (left) and NpHR group (right). G Statistics of the change of BSR before and during optical suppression, n = 6 per group. *P < 0.05, **P < 0.01, data are graphed as mean ± SD

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BMC Anesthesiology

ISSN: 1471-2253

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