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Fig. 1 | BMC Anesthesiology

Fig. 1

From: Selective optogenetic modulation of the PBN terminals in the lateral hypothalamic area and basal forebrain regulates emergence from isoflurane anesthesia in mice

Fig. 1

Optical stimulation of PBN-LH projections accelerates emergence from isoflurane anesthesia. A Schematic of excitatory optogenetic virus (ChR2) injection into the PBN and optical implant into the LH in mice. B Histological and fluorescence image showing the virus expression in the PBN. C Representative images (left) and quantification (right) of EYEP (green), c-Fos (red), and DAPI (blue) colabeled in the LH, n = 5 per group. D Schematic showing the protocol of optogenetic activation of PBN-LH projections during isoflurane anesthesia. E and F LORR time and RORR time of mice with optogenetic activation of PBN-LH projections, n = 8 per group. G Representative EEG traces (above) and corresponding power spectra (below) optical activation under 1.5% isoflurane anesthesia in the EYEP (left) and ChR2 group (right). H Statistics of the change of BSR before and during optical stimulation, n = 5 per group. **P < 0.01, ***P < 0.001, ****P < 0.0001, data are graphed as mean ± SD

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BMC Anesthesiology

ISSN: 1471-2253

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