Fig. 4
The change of threshold stimulus intensity and ISIs in the neurons of the BF (HDB) caused by DEX was eliminated by PHEN. (A) is the staining diagram of α2 adrenergic receptor in the BF(HDB). Blue and green represented DAPI and α2 adrenergic receptor. Scale bar: 200 μm (Left), 20 μm (Right). (B) Statistical data of threshold stimulus intensity in the neurons of the BF (HDB) after DEX and DEX + PHEN administration (one-way ANOVA followed by Tukey’s test,, Baseline vs. DEX, DEX vs. DEX + PHEN, n = 5 neurons from 5 mice). (C)-(E) Statistical data of ISIs (ISI1 − 2, ISI2 − 3, ISI3 − 4) in the neurons of the BF (HDB) before, during and after DEX administration (one-way ANOVA followed by Tukey’s test, Baseline vs. DEX, DEX vs. Wash, n = 8 neurons from 8 mice)