Fig. 5

Inhibition of astrocytes in the BF region delays anesthesia-induced loss of consciousness and promotes recovery. (A) Diagram of astrocyte inhibitory experiment. (B) Time of loss of righting reflex (LORR) with isoflurane exposure (n = 8). (C) Time of recovery of righting reflex (RORR) after isoflurane exposure ceased (n = 8). (D, F) Representative power ratio of EEG recording of isoflurane anesthesia during anesthesia maintenance (D), and recovery (F) periods. Blue line represents aCSF group; green line represents L-a-AAA group. (E, G) Comparison of EEG power ratios of different frequency bands (delta, theta, alpha, beta, and gamma power bands) in different periods between the aCSF and L-a-AAA groups (aCSF group, n = 7; L-α-AAA group, n = 8). Asterisks indicate significant differences (^*P < 0.05, ^**P < 0.01)