Fig. 4

Activation of astrocytes in the basal forebrain region alters the power ratio of electroencephalogram (EEG) spectrum during anesthesia maintenance and recovery period. (A) Representative raw traces of EEG during different anesthesia periods. (B) Representative power spectrograms of EEG from two different groups. (C, E, G, I) Representative power ratio of EEG recording of isoflurane anesthesia during wake (C), induction (E), anesthesia maintenance (G), and recovery (I) periods. Blue line represents the hM3Dq mice injected with saline; green line represents the hM3Dq mice injected with clozapine N-oxide (CNO). (D, F, H, J) Comparison of EEG power ratios of different frequency bands (delta, theta, alpha, beta, and gamma) in different periods between the hM3Dq-Saline and hM3Dq-CNO groups (n = 4). The asterisk indicates a significant difference (^*P < 0.05, ^**P < 0.01).