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Fig. 1 | BMC Anesthesiology

Fig. 1

From: Activation of astrocytes in the basal forebrain in mice facilitates isoflurane-induced loss of consciousness and prolongs recovery

Fig. 1

hM3Dq-mCherry is specifically expressed in astrocytes, but not in neurons. (A) Adeno-associated viral vectors with Cre-dependent expression of hM3Dq-mCherry or mCherry proteins (rAAV-EF1α-DIO-hM3Dq-mCherry or rAAV-EF1α-DIO-mCherry) are bilaterally injected into the basal forebrain of Aldh1l1-CreERT2 mice. (B) Representative image of viral expression in the basal forebrain of Aldh1l1-CreERT2 mice. (C) Overlay of hM3Dq-mCherry expression in the basal forebrain. Color depth represents the number of mice. (D) hM3Dq (red) is specifically expressed in astrocytes in the basal forebrain (scale bar, 50 μm). White arrows indicate the co-labeled of GFAP and mCherry. (E and F) hM3Dq is expressed in 87.54% of astrocytes (19 slices from 5 mice) (E), with the specificity of 94.62% (19 slices from 5 mice) (F). (G) Co-localization between mCherry and NeuN, the neuronal nuclear marker, is rarely detected (scale bar, 100 μm)

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BMC Anesthesiology

ISSN: 1471-2253

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