Fig. 3

NLRP3 regulated the activation of the NLRP3 inflammasome as well as the degradation of cell junction proteins after mechanical ventilation, but aerobic exercise alleviated these changes. (A) Western blotting was used to determine the expression of NLRP3, PKCɑ and occludin. GAPDH was used as a reference. When we performed WB, the blots were cut prior to hybridization with antibodies, and we developed strips of different proteins on the same PVDF film separately. The unedited blots are shown in the Supplementary File. (B) *p < 0.01 versus the C group, #p < 0.01 versus the MV group, &p < 0.01 versus the EX group. C, control group; MV, mechanical ventilation group; EX, 5-week aerobic training intervention group; EX + MV, 5-week aerobic training intervention plus mechanical ventilation group. The data are representative of three independent experiments