Fig. 2

Aerobic exercise inhibited the assembly and activity of the NLRP3 inflammasome induced by mechanical ventilation. (A) The protein levels of P-PKCɑ, ASC, procaspase-1, caspase-1, pro-IL-1β and IL-1β were detected by Western blotting. When we performed WB, the blots were cut prior to hybridization with antibodies, and we developed strips of different proteins on the same PVDF film separately. The unedited blots are shown in the Supplementary File. (B-G) *p < 0.01 versus the C group, #p < 0.01 versus the MV group. C, control group; MV, mechanical ventilation group; EX, exercise group (only received 5 weeks of aerobic exercise training); EX + MV, exercise + mechanical ventilation group (underwent 5 weeks of aerobic exercise running plus mechanical ventilation). The data are representative of three independent experiments