Fig. 1

Mechanical ventilation increased the levels of NLRP3, P-PKCɑ, and PKCɑ, but these changes were reversed by bisindolylmaleimide I. Inhibition of PKC expression prevented the activation of NLRP3. (A) The protein levels of NLRP3, PKCɑ and P-PKCɑ were measured by Western blotting. When we performed WB, the blots were cut prior to hybridization with antibodies, and we developed strips of different proteins on the same PVDF film separately. The unedited blots are shown in the Supplementary File. (B, C, D) *p < 0.01 versus the C group, #p < 0.01 versus the MV group. C, control group; B, group pretreated with bisindolylmaleimide I 0.02 mg/kg MV, mechanical ventilation group; B + MV, group pretreated with bisindolylmaleimide I 0.02 mg/kg for 1 h before mechanical ventilation (4 h). (E-F) The pathological changes in lung tissues were determined by HE staining (magnification 400×) and lung injury scores. *p < 0.01 versus the C group, ^#p < 0.01 versus the B group, and ^&p < 0.01 versus the MV group. The scale bar represents 16 μm. The data are representative of three independent experiments