Fig. 4

BDNF was the downstream target of miR-10b-5p. A The downstream targets of miR-10b-5p were predicted and screened through the TargetScan and miRDB database. B The binding relationship between miR-10b-5p and BDNF was verified using dual-luciferase assay. C-D BDNF expression in HT22 and SH-SY5Y cells was detected using RT-qPCR and Western blot. The cell experiment was repeated 3 times independently. Data are presented as mean ± standard deviation. Data in panel B were analyzed using two-way ANOVA, and data in panels C-D were analyzed using one-way ANOVA, followed by Tukey's multiple comparisons test, ^*p < 0.05