Fig. 3

miR-10b-5p overexpression reversed the protection of dexmedetomidine pretreatment on ropivacaine-induced neurotoxicity. miR-10b-5p-mimic (miR-mimic) was transfected into HT22 and SH-SY5Y cells, with mimic-NC as negative control. A Transfection efficiency was measured using RT-qPCR, followed by a combined experiment with Dex-pretreated cells. B Cell viability was measured using CCK-8 assay. C LDH release was measured using LDH detection kit. D Apoptosis was measured using flow cytometry. The cell experiment was repeated 3 times independently. Data are presented as mean ± standard deviation. Data in panel A were analyzed using t test; data in panel B were analyzed using two-way ANOVA, and data in panels C-D were analyzed using one-way ANOVA, followed by Tukey's multiple comparisons test, ^&p < 0.05, compared with the Ropi + DEX group, ^*p < 0.05