Table 1 Primers and probes used for real-time polymerase chain reaction
PCR Assay (product length, annealing temperature) | Oligonucleotide Sequence (5′-3′) | Gene Bank Number |
|---|---|---|
Cyclophilin A (PPIA), [212 bp, 58°C] | Forw: 5′-GCGTCTSCTTCGAGCTGTT-3′ Rev: 5′-RAAGTCACCACCCTGGCA-3′ FL: 5′-GCTCTGAGCACTGGRGAGAAAGGA-FL Cy5: Cy5-TTGGCTATAAGGGTTCCTCCTTTCACAG-Phos | NM_008907 |
TNF-α (Tnfa), [212 bp, 62°C] | Forw: 5′-TCTCATCAGTTCTATGGCCC-3′ Rev: 5′-GGGAGTAGACAAGGTACAAC-3′ | NM_ 008361 |
IL-1β (Il1b), [348 bp, 55°C] | Forw: 5′-59-GTGCTGTCGGACCCATATGAG-3′ Rev: 5′-CAGGAAGACAGGCTTGTGCTC-3′ FL: 5′-TAATGAAAGACGGCACACCCACCC-FL Cy5: Cy5-TTGGCTATAAGGGTTCCTCCTTTCACAG-Phos | NM_008361 |
IL-6 (Il6), [141 bp, 55°C] | Forw: 5′-GAGGATACCACTCCCAACAGACC-3′ Rev: 5′-AAGTGCATCATCGTTGTTCATACA-3′ | NM_031168 |
iNOS (NOS2), [312 bp, 58°C] | Forw: 5′-TGTGTCAGCCCTCAGAGTAC-3′ Rev: 5′-CACTGACACTYCGCACAA-3′ R640: Red-GCTCCTCCCAGGACCACACCC-Phos FL: 5′-GAAGCCCCGCTACTACTCCATC-FL | NM_010927 |