Fig. 2

Neurofunctional recovery. A Neurological function was examined 3 days after experimental TBI using the neurological severity score (0 point = no impairment, 15 points = maximal impairment). All mice exhibited moderately impaired neurological function after trauma. Mice treated with low-dose midazolam exhibited significantly impaired neurological function compared with mice in the vehicle group. B effect of midazolam or midazolam plus flumazenil treatment on motor function was evaluated using the rotarod test. The performance of the high-dose midazolam group was significantly poorer than that of the vehicle group (normal saline, NaCl 0.9%), **P < 0.01, *P < 0.05, n = 9 mice for the Mida HD and the Mida HD + Fluma group, n = 11 mice for the Mida LD and the vehicle group; data are presented as mean ± SD; Mida LD, midazolam low-dose group; Mida HD, midazolam high-dose group; Mida HD + Fluma, midazolam high-dose plus flumazenil.