Fig. 1

Righting reflexes and brain lesiom volume. A Timeline of the study. B Loss of righting reflex (LORR) was measured by an investigator blinded to the group allocation. There was a dose-dependent increase in LORR. The vehicle group and the group that received midazolam and flumazenil simultaneously did not show any LORR. C, 72 h after injury, the cresyl-violet-stained sections obtained from mice exposed to midazolam or midazolam plus flumazenil application at 24 h after trauma were evaluated for brain damage and compared with the vehicle group (normal saline, NaCl 0.9%). Brain lesion volume was not affected by posttraumatic midazolam or flumazenil administration. Representative cresyl-violet-stained sections at the coronal plane from 1.70 mm anterior to the bregma, 0.46 mm posterior to the bregma, and 1.46 mm posterior to the bregma; n = 9 mice for the Mida HD and the Mida HD + Fluma group, n = 11 mice for the Mida LD and the vehicle group. *P < 0.05; data are presented as mean ± SD; Mida LD, midazolam low-dose group; Mida HD, midazolam high-dose group; Mida HD + Fluma, midazolam high-dose plus flumazenil.