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Fig. 4 | BMC Anesthesiology

Fig. 4

From: miR-424 inhibits apoptosis and inflammatory responses induced by sevoflurane through TLR4/MyD88/NF-κB pathway

Fig. 4

TLR4 is a direct target of miR-424 in PC12 cells. A. Diagram of complementary pairing between miR-424 and wild-type TLR4 or mutant TLR4 3′-UTR. B. Interaction between miR-424 and wild-type or mutant TLR4 was detected by dual luciferase report assays. C. Validation of miR-424 expression in PC12 cells with RT-PCR. D–G. Western blot was used to detect TLR4/MyD88/NF-κB signaling pathway-associated proteins. Images were quantified by ImageJ software, and the ratios of TLR4/GAPDH, MyD88/GAPDH, and p-NF-kB/NF-kB were calculated. *p < 0.05 vs control; #p < 0.05 vs Sev + NC + pcDNA; @p < 0.05 vs Sev + Mimic+pcDNA; %p < 0.05 vs Sev + NC + TLR4

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BMC Anesthesiology

ISSN: 1471-2253

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