Fig. 4

The effects of PI3K/AKT signaling on cell survival of breast cancer cells with OPRK1 knockdown. A-B MDA-MB-231 and MCF-7 cells transfected with OPRK1 siRNA were treated with Recilisib for 24 h, and the protein expressions of p-AKT, AKT, p-PI3K, PI3K were determined by western blot. GAPDH was used as loading control. C MDA-MB-231 cells transfected with OPRK1 siRNA were treated with Recilisib or Buparlisib for 24 h, and the cell viability was determined by MTT assay. D MDA-MB-231 cells transfected with OPRK1 siRNA were treated with Buparlisib for 24 h, and the cell death rates were determined by Annexin V/PI staining and flow cytometry