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Table 3 Primer sequences for RT-qPCR

From: Protective effects of dexmedetomidine on cerebral ischemia/reperfusion injury via the microRNA-214/ROCK1/NF-κB axis

Targets

Sequences (5′-3′)

miR-214

F: AGAGTTGTCATGTGTCT

R: GAACATGTCTGCGTATCTC

ROCK1

F: CACGCCTAACTGACAAGCACCA

R: CAGGTCAACATCTAGCATGGAAC

SOX4

F: GATCTCCAAGCGGCTAGGCAAA

R: GATCTCCAAGCGGCTAGGCAAA

SEMA4C

F: GGAGTATGACTGCTATTCCGAGC

R: ACACCAACCGAGCCTTCAGGAA

PPTC7

F: GCGGTTAGTGAAAGAAGGACGC

R: TTCTGTCCAGCACCACGATGCA

U6

F: CTCGCTTCGGCAGCACAT

R: TTTGCGTGTCATCCTTGCG

GAPDH

F: CATCACTGCCACCCAGAAGACTG

R: ATGCCAGTGAGCTTCCCGTTCAG

  1. Notes: RT-qPCR Reverse transcription quantitative polymerase chain reaction, F Forward, R Reverse, miR-214 microRNA-214, ROCK1 Rho-associated kinase 1, SOX4 SRY-box transcription factor 4, SEMA4C Semaphorin 4C, PPTC7 Protein phosphatase targeting COQ7, GAPDH Glyceraldehyde-3-phosphate dehydrogenase
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BMC Anesthesiology

ISSN: 1471-2253

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