Table 1 Grouping for experimental animals
Group (n = 10) | Surgerical procedures |
|---|---|
sham | Procedures for anesthesia were the same as that for the MCAO group, except for the occlusion of middle cerebral artery |
DEX | Based on the sham group, DEX was intravenously administered at a loading dose of 1 μg/kg at the very beginning of the surgery, and was then administered at 0.05 μg/kg/min for the next two hours |
MCAO | MCAO modeling |
MCAO + DEX | Simultaneous treatment of MCAO modeling and DEX |
NC inhibitor/miR-214 inhibitor | Based on the operation of MCAO + DEX, NC inhibitor/miR-214 inhibitor (80 nM) with invivofectamine was administered via intracerebroventricular infusion half an hour before surgery |
oe-NC/oe-ROCK1 | Based on the operation of MCAO + DEX, oe-NC/oe-ROCK1 (100 nM) with invivofectamine was administered via intracerebroventricular infusion half an hour before surgery |