Fig. 2

Effects of desflurane and sevoflurane anesthesia on the CD4 + CD25 + FoxP3+ Tregs (n = 20 per group). a Representative gating strategy of Treg enumeration. b The percentage of Treg in the CD4 + cell population. c The absolute number of white blood cells (left), and the calculated numbers of CD4 + cells (center) and Treg (right), in peripheral blood (details in the Method section). The result showed that the LDKT patients who received desflurane anesthesia, but not sevoflurane, had significantly increased Tregs in the peripheral blood at 24-h post-exposure. All experiments were performed in triplicate. Des, desflurane; NS, not significant; Sevo, sevoflurane