Fig. 4
Co-expression of GPR30 and GABAAα4 (a), GPR30 and GABAAβ1 (b), as well as GPR30 and GABAAδ (c) in periaqueductal gray (PAG) after incision surgery. Double immunofluorescence staining for confocal laser scanning microscopy showing a transverse section of PAG. In Group S + I, GPR30/GABAAα4 (c, f), GPR30/GABAAβ1 (i, l), and GPR30/GABAAδ (o, r), simultaneously immunopositive in neurons in PAG were co-localized, as indicated by yellow overlay. Scale bar is 100 μm in a–c, g–i, and m–o, and 20 μm in d–f, j–l, and p–r