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Fig. 1 | BMC Anesthesiology

Fig. 1

From: GPR30 receptor promotes preoperative anxiety-induced postoperative hyperalgesia by up-regulating GABAA-α4β1δ subunits in periaqueductal gray in female rats

Fig. 1

The schematic representation of the experimental design and time schedule of the protocol. All the female rats were subjected to bilateral ovariectomy and cannula implantation in the periaqueductal gray on day 1, and received estrogen replacement for 10 days. The single prolonged stress was administered on day 7, and G1/G15 microinjection was once per day from day 6 to 8 after the estrogen replacement. Behavioral tests were conducted on day 6 prior to the incision surgery, and on day 8, 9, and 10 after the surgical incision. Samples were collected for immunofluorescence and western blotting analysis on day 10 after behavioral tests

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BMC Anesthesiology

ISSN: 1471-2253

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