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Fig. 3 | BMC Anesthesiology

Fig. 3

From: Propofol attenuated TNF-α-modulated occludin expression by inhibiting Hif-1α/ VEGF/ VEGFR-2/ ERK signaling pathway in hCMEC/D3 cells

Fig. 3

The phosphorylation of ERK was involved in the effects of propofol and TNF-α on occludin expression in hCMEC/D3 cells. a Left side is the protein expression of p-ERK, p-p38MAPK, ERK and p38MAPK in hCMEC/D3 cells that treated with TNF-α and propofol. Right side is the plot of normalized ratios of optical densities. b The hCMEC/D3 cells were treated with TNF-α, propofol, and ERK inhibitor (LY3214996, 5 μM). Left side is the protein expression of p-ERK, ERK, and occludin. Right side is the plot of normalized ratios of optical densities. β-actin was served as internal loading control. Data were represented as the mean ± SD. *p < 0.05, compared with the control group. #p < 0.05, compared with TNF-α treatment group. Each assay was performed in five replicates

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BMC Anesthesiology

ISSN: 1471-2253

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