Fig. 2

Propofol could inhibit TNF-α-modulated occludin expression in hCMEC/D3 cells. a The hCMEC/D3 cells were pretreated with different concentrations of propofol (5, 10, 25, 50 μM) for 2 h, followed by TNF-α treatment (10 ng/ml, 4 h). Left side is the image of a representative Western blot for occludin. Right side is the normalized ratios of optical densities. b DMSO served as solvent for propofol in this study. Left side is the protein expression of occludin in hCMEC/D3 cells that treated with TNF-α, propofol and DMSO (0.01%). Right side is the normalized ratios of optical densities. β-actin was served as internal loading control. Data were represented as the mean ± SD. *p < 0.05, compared with the control group. #p < 0.05, compared with TNF-α treatment group. Each assay was performed in five replicates