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Fig. 2 | BMC Anesthesiology

Fig. 2

From: Antiarrhythmic effect of sevoflurane as an additive to HTK solution on reperfusion arrhythmias induced by hypothermia and ischaemia is associated with the phosphorylation of connexin 43 at serine 368

Fig. 2

HE and TUNEL staining in myocardial tissue (400×). Apoptotic nuclei were identified by TUNEL staining (red), and total nuclei were identified by DAPI counterstaining (blue). a Part of the cardiac muscle fibres appeared broken, swollen and disordered; the black arrow indicates fractured cardiac muscle fibres in the control. b Pathological damage to the myocardium was ameliorated in donor hearts stored in Sevo-HTK solution. c and d Show that the number of apoptotic nuclei increased in the Control group. e and f Show that the number of apoptotic nuclei decreased in the Sevo-HTK group. g Sevoflurane as an additive appreciably lowered the number of apoptotic cells. h Difference between the graft re-beating times in the two groups

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