Fig. 2

Immunohistochemical staining revealed that the protein levels of NMDAR and BDNF in hippocampus tissue. A. NMDAR and BDNF were expressed abundantly both in the nucleus and cytoplasm in the PND group, the improvement effect of the PNK group was inhibited compared to PN, the improvement effect of the PMD group was inhibited compared PD group. B. The mRNA expression of NMDAR, proBDNF, mBDNF, TrkB, ERK1 and CREB were tested by RT-PCR. The mRNA levels of NMDAR, mBDNF and TrkB were lower in the P group than those in the NS group, while the level of proBDNF was up-regulated in the P group compared to NS group. However, there were no obvious different expression of ERK1and CREB between the NS group and the others. C, F. The protein phosphorylation of NMDAR, proBDNF, mBDNF, TrkB, ERK1 and CREB were determined by Western blot. The phosphorylation levels of NMDAR, TrkB, ERK1 and CREB in the PND group were highest, while P group were the lowest. D, E. The ratio of p-NMDAR and NMDAR, the ratio of p-TrkB andTrkB. *versus NS group, P < 0.05; #versus P group, P < 0.05; &versus PN group, P < 0.05; $versus PD group, P < 0.05