Fig. 2

The expression of related proteins exhibited time- and dose-dependent changes. Western blotting was performed to analyse the expression of TREK-1 (a, N = 4) and BDNF (b, N = 4) in astrocytes following different isoflurane doses and exposure time. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays were used to calculate the relative TREK-1 (c and d, N = 5) and BDNF (e and f, N = 4) mRNA levels in astrocytes following different isoflurane doses and exposure time. Data are presented as relative to Con group. (a, c and e) Astrocytes were treated with different doses of isoflurane (0%, 1.2%, 2.4%, and 3.6%) for 9 h. (b, d and f) Astrocytes were treated with 2.4% isoflurane for different durations (0, 3, 6, and 9 h). ISO, isoflurane. The data are presented as the mean ± SEM. ^* P < 0.05, ^** P < 0.01. The P values were determined by one-way ANOVA (a, b, c, d, e and f) with correction for multiple comparisons