Fig. 5

Microglia viewed in the CC region from the saline and LPS injection groups. a, anti-Iba-1 immunostained CC area from the saline injected animals (n = 3). b and c, high amplification of framed areas in a, showing resting microglia with thin somata and delicate pseudopodia in white matter. d, anti-Iba-1 immunostained CC area from LPS group at the day 3 (n = 3). e and g, high magnification images from the framed areas in d, showing Iba-1 stained cells with hypertrophic soamta and amoeboid appearance. g, anti-Iba-1 immnuostained CC region from LPS group (n = 3) at the day 6. h to j, high magnification of the framed areas in the g, showing that 3 days after termination of the LPS injection, microglia are still visualized as amoeboid appearance but with further hypertrophied somata. Scale bar in a is 150 μm. scale bars in d and g are 100 μm, in b and C are 25 μm, and in e, f, h, i and j are 20 μm. LV, lateral ventricle. BG, basal ganglion