Fig. 4

RIPC has no effect on mitochondrial hexokinase. The amount of mitochondrial bound hexokinase II (mtHKII) and HK activity were measured in atrial tissue samples. Representative images of mtHKII/VDAC determined by western blot (a). HKII levels in mitochondria did not differ between control and RIPC treated patients (b). No correlation between cTnT area under the curve (AUC) and mtHKII levels was observed (c). HK activity in the mitochondria corrected for the amount of protein (d) or citrate synthase (CS) activity (e) did not differ between groups. mtHKII: mitochondrial bound hexokinase II, HK: hexokinase, cTnT: cardiac troponin T, AUC: area under the curve, CS: citrate synthase. Single values and median (a and b) or mean (d and e). Control n = 14, RIPC n = 10