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Fig. 6 | BMC Anesthesiology

Fig. 6

From: Pretreatment but not subsequent coincubation with midazolam reduces the cytotoxicity of temozolomide in neuroblastoma cells

Fig. 6

Effect of pretreatment with midazolam to the G2/M arrest induced by temozolomide. The cell cycle was analysed by flow cytometric measurement after staining of cells with propidium iodide. The percentage of cells with a diploid DNA content indicating the G2/M phase of the cell cycle was detected to discriminate the expected effect of temozolomide. Whereas no G2/M arrest was detected after 24 h, temozolomide (100 μM) induced a significant increase of G2/M positive cells after 48 h. This effect was not attenuated by pretreatment with midazolam (16 μM) (n = 5; data are expressed as mean ± SD; * = P < 0.05)

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BMC Anesthesiology

ISSN: 1471-2253

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