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Fig. 1 | BMC Anesthesiology

Fig. 1

From: Pretreatment but not subsequent coincubation with midazolam reduces the cytotoxicity of temozolomide in neuroblastoma cells

Fig. 1

Midazolam-induced hormesis and toxicity in neuroblastoma (SHEP) cells. Cell viability of neuroblastoma (SHEP) cells was measured with the XTT assay to investigate cell viability after incubation for 24 h with increasing concentrations of midazolam. The "Zero Equivalent Point" (ZEP) describes the point of reversal of response and defines the concentration of midazolam, which is characterised by the transition from protective to toxic effects [17]. The ZEP of midazolam was 79.4 μM. A significant increase of cell viability in comparison to control was detected after incubation with 2, 4, 8, and 16 μM, whereas a significant decline was measured after incubation with 128, 256 and 512 μM midazolam (n = 8; * = P < 0.05 compared to control (0 μM midazolam); data are expressed as mean ± SD)

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BMC Anesthesiology

ISSN: 1471-2253

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