Figure 3

Lung immunohistochemistry for ICAM-1 protein expression in lung tissues. NV = normal-pressure ventilation with normocapnia; NC = high-pressure ventilation with normocapnia; MHA = moderate hypercapnic acidosis; SHA = severe hypercapnic acidosis (A) Immunohistochemical staining of ICAM-1 in the lung. Sections were stained with a brown DAB color-developing agent and counterstained with hematoxylin. The presence of brown granules in the nucleus was defined as a positive cell. All panels represent a 400x magnification. (B) Scatter plot of ICAM-1-positive (+) cells (%) in lung tissue. Semi-quantitative analysis of ICAM-1 indicated that hypercapnic acidosis decreased the expression of ICAM-1. Horizontal bars represent the median. n = 4 for each group. ^ap <0.05 versus the NV group; ^bp < 0.05 versus the NC group.