DAPT (an inhibitor of notch signaling pathway) administered before appearance of pain sensitivity significantly prevents the development of mechanical allodynia in SNI-induced neuropathic pain. A single administration of DAPT (50 μM, 10 μL, i.t.) was 0.5 h before SNI operation. The mechanical mechanical paw withdrawal threshold (PWT) was measured at 24 h before (baseline) as well as 7 d, 14 d, 21 d and 28 d after SNI or Sham operation. The data represent mean ± SD (n = 6 per group). *P < 0.05 versus Sham group; †P < 0.05 versus SNI group. SNI: spared nerve injury; BL: baseline; g: gram; d: day.