High throughput modular chambers for rapid evaluation of anesthetic sensitivity

BMC Anesthesiology

Table 3 Anesthetic Effects upon Mouse Body Temperature

Condition	Average	Standard Error	Number of Mice
Baseline Temperature Prior to placement in chambers (FGF = 0) No anesthetic exposure No active warming	37.3	0.1	109
Temperature after 2 hr exposure to FGF = 100 ml/min oxygen No anesthetic exposure No active warming	36.6	0.1	24
Temperature after 2 hr exposure to FGF = 100 ml/min oxygen Anesthetic = Isoflurane 1.25% No active warming	25.4	2.3	30
Temperature after 2 hr exposure to FGF = 100 ml/min oxygen Anesthetic = Isoflurane 1.25% Apparatus in water bath set to 33°C	33.0	0.2	10
Temperature after 2 hr exposure to FGF = 100 ml/min oxygen Anesthetic = Isoflurane 1.25% Apparatus in water bath set to 36°C	36.2	0.2	10
Temperature after 2 hr exposure to FGF = 100 ml/min oxygen Anesthetic = Isoflurane 1.25% Apparatus in water bath set to 40°C	39.8	0.2	11
Temperature after 2 hr exposure to FGF = 100 ml/min oxygen No Anesthetic Exposure Apparatus in water bath set to 37°C	36.5	0.1	24
Temperature after 2 hr exposure to FGF = 100 ml/min oxygen Anesthetic = Isoflurane 1.25% Apparatus in water bath set to 37°C	36.6	0.1	16

Temperature measurements at baseline and following 2 hours of exposure to 100 ml/min oxygen fresh gas flow (FGF) in controlled environment chambers (250 ml volume apiece) in the presence or absence of 1.25% isoflurane and varying degrees of warming obtained by submerging controlled environment chambers in a water bath. Room temperature varied between 20–22°C.

ISSN: 1471-2253