Do different anesthesia regimes affect hippocampal apoptosis and neurologic deficits in a rodent cardiac arrest model?

Bendel, Stepani; Springe, Dirk; Pereira, Adriano; Grandgirard, Denis; Leib, Stephen L; Putzu, Alessandro; Schlickeiser, Jannis; Jakob, Stephan M; Takala, Jukka; Haenggi, Matthias

doi:10.1186/1471-2253-15-2

Table 2 Results of the general health and neurologic tests

From: Do different anesthesia regimes affect hippocampal apoptosis and neurologic deficits in a rodent cardiac arrest model?

		Medetomidine/ketamine	Sevoflurane/fentanyl	p between groups Mann–Whitney test
Weight [g]	BL	385 [IQR 357 – 413]	380 [IQR 360 – 426]	p = 0.89
	day 1	375 [IQR 353 – 415]	367 [IQR 343 – 420]
	day 2	360 [IQR 322 – 400]	355 [IQR 342 – 387]
	day 3	359 [IQR 322 – 413]	359 [IQR 348 – 360]
	day 4	369 [IQR 320 – 417]	349 [IQR 343 – 351]
	day 5	370 [IQR 325 – 421]	350 [IQR 319 – 360]
Friedman & (BL, day 1 and last day, all animals)		p < 0.01	p < 0.01
Friedman^% (day 2–5, only animals surviving all days)		p = 0.14	p = 0.58
NDS	BL	0 [IQR 0 – 0]	0 [IQR 0 – 0]	p = 0.34
	day 1	10 [IQR 5 – 14]	5 [IQR 3 – 10]
	day 2	5 [IQR 0 – 5]	0 [IQR 0 – 5]
	day 3	0 [IQR 0 – 0]	0 [IQR 0 – 15]
	day 4	0 [IQR 0 – 0]	0 [IQR 0 – 15]
	day 5	0 [IQR 0 – 0]	0 [IQR 0 – 15]
Friedman & (BL, day 1 and last day, all animals)		p < 0.01	p < 0.01
Friedman^% (day 2–5, only animals surviving all days)		p = 0.93	p = 0.07
OFT [n]	BL	94 [IQR 75 – 110]	91 [IQR 64 – 114]	p = 0.89
	day 1	107 [IQR 26 – 147]	102 [IQR 52 – 163]
	day 2	106 [IQR 55 – 130]	114 [IQR 81 – 158]
	day 3	35 [IQR 17 – 93]	90 [IQR 63 – 135]
	day 4	35 [IQR 9 – 66]	42 [IQR 13 – 148]
	day 5	20 [IQR 11 – 33]	66 [IQR 23 – 138]
Friedman & (BL, day 1 and last day, all animals)		p = 0.23	p = 0.76
Friedman^% (day 2–5, only animals surviving all days)		p = 0.04	p = 0.73
rears [n]	BL	23 [IQR 17 – 29]	19 [IQR 16 – 25]	p = 0.21
	day 1	2 [IQR 0 – 13]	5 [IQR 1 – 12]
	day 2	12 [IQR 1 – 14]	11 [IQR 4 – 19]
	day 3	7 [IQR 3 – 11]	6 [IQR 3 – 11]
	day 4	6 [IQR 4 – 8]	5 [IQR 0 – 13]
	day 5	5 [IQR 3 – 7]	4 [IQR 0 – 13]
Friedman & (BL, day 1 and last day, all animals)		p < 0.01	p = 0.02
Friedman^% (day 2–5, only animals surviving all days)		p = 0.21	p = 0.18
VPT	BL	0 [IQR 0 – 0]	0 [IQR 0 – 0]	p = 0.70
	day 1	2 [IQR 1 – 2]	2 [IQR 2 – 2]
	day 2	1 [IQR 1 – 2]	1 [IQR 1 – 2]
	day 3	0 [IQR 0 – 1]	1 [IQR 1 – 1]
	day 4	0 [IQR 0 – 0]	0 [IQR 0 – 2.0]
	day 5	0 [IQR 0 – 1]	0 [IQR 0 – 1.0]
Friedman & (BL, day 1 and last day, all animals)		p < 0.01	p < 0.01
Friedman^% (day 2–5, only animals surviving all days)		p = 0.16	p = 0.43
TRT [sec]	BL	18.5 [IQR 11.5 – 23.5]	11.0 [IQR 9.5– 19.5]	p = 0.12
	day 1	120 [IQR 120 – 120]	120 [IQR 93.5 – 120]
	day 2	55.0 [IQR 15.0 – 120]	48.0 [IQR 29.5 – 120]
	day 3	32.0 [IQR 11.8 – 95]	62.0 [IQR 19.0– 120]
	day 4	46.0 [IQR 21.0 – 102.5]	28.0 [IQR 19.0– 120]
	day 5	24.0 [IQR 12.3 – 96.5]	36.0 [IQR 18.0– 112]
Friedman & (BL, day 1 and last day, all animals)		p < 0.01	p < 0.01
Friedman^% (day 2–5, only animals surviving all days)		p = 0.65	p = 0.80

Friedman test including baseline, first and last day assessments (all animals)&, and including assessment from day 2 to 5, respectively (only animals with longest survival)^%. No significant differences could be found between both anesthesia groups at baseline, and global (BL, day 1 and last day) Friedman’s tests (time effect) did not diverge. The only difference found is a reduction in the numbers of squares crossed in the open field test during the recovery phase after day 2 in the med/ket group, compared to the sev/fnt group, in which the animals remained mobile.
NDS: Neuro Deficit Score; OFT: Open Field Test (numbers of squares crossed in the open field); rears: number of rears in the open field; VPT: Vertical Pole Test, TRT: Tape Removal Test.

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ISSN: 1471-2253

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