Figure 4

Example of injury to the hippocampus (cresyl violet staining). Left: sham animal, right: 8 minutes cardiac arrest. The 4 different slides of one hemisphere are showing the different sections of the CA1 segment of the hippocampus while moving from rostral to caudal (starting up-left, up-right, down-left and down-right). Delineating the border of the CA1 segments is difficult in the caudal sections because of the curvature of the hippocampus. The inlays demonstrate the shrunken and pyknotic neurons, resulting in a diminished cell layer of CA1 in the cardiac arrest animal (400x). Histomorphometric analysis of the CA1 segment was performed by 1. cell count and 2. automated surface area calculation (details see Methods). The sham animal was operated in the pilot phase, and received complete surgery under sev/fnt anesthesia, but was not subjected to cardiac arrest.