Figure 7

Effect of Src inhibitor on LPS-induced ICAM-1 expression in HLMVEC. (A) Representative Western blot of HLMVEC ICAM-1 (total ICAM-1, row 1) and β-actin (row 2) after treatment of the cells with either vehicle (dimethylsulfoxide, DMSO 0.1%), Src inhibitor PP2 (pretreatment for 30 minutes at a concentration of 10 μM), LPS (4 μg/ml), or LPS following pretreatment with PP2. (B) Quantitative densitometry analysis of the ratio of total ICAM-1 over β-actin. Values shown are mean ± SEM. ^# p < 0.05 vs. vehicle (control), *p < 0.05 compared to LPS alone.