Figure 3
Effect of ropivacaine on phosphorylation and expression of Src, ICAM-1, and caveolin-1 in the mouse lung during ALI as induced by LPS. Src protein tyrosine kinase (Src) and its substrates intercellular adhesion molecule-1 (ICAM-1) and caveolin-1 in mouse lung homogenates were assessed after induction of acute lung injury with bacterial lipopolysaccharide (LPS) in C57BL/6 wild type mice. (A.) ( i.) Representative Western Blot of lung homogenates probing for Src tyrosine kinase, phosphorylated at tyrosine 418 (pTyr418-Src), total Src as well as β-actin. Quantitative densitometry analysis of Western blots showing ( ii.) the ratio of pTyr418-Src over total Src compared to control animals (set as 1.0, white bars; N = 5/group), and ( iii.) Src expression as a ratio of total Src over β-actin compared to control animals (set as 1.0, white bars; N = 5/group). ( B.) ( i.) Representative Western Blot of lung homogenates probing for ICAM-1, phosphorylated at tyrosine 518 (pTyr518-ICAM-1), total ICAM-1, as well as β-actin. Quantitative densitometry analysis showing ( ii.) the ratio of pTyr518-ICAM-1 over total ICAM-1 compared to control animals (set as 1.0, white bars; N = 5/group), ( iii.) ICAM-1 expression as the ratio of total ICAM-1 over β-actin compared to control animals (set as 1.0, white bars; N = 5/group). ( C.) ( i.) Representative Western Blot of lung homogenates probing for caveolin-1, phosphorylated at tyrosine 14 (pTyr14-Cav-1), total Cav-1 as well as β-actin. Quantitative densitometry analysis showing ( ii.) the ratio of pTyr14 Cav-1 over total Cav-1 compared to control animals (set as 1.0, white bars; N = 5/group), and ( iii.) Cav-1 expression as a ratio of total Cav-1 over β-actin compared to control animals (set as 1.0, white bars; N = 5/group). All values shown are mean ± SD; # p < 0.05 vs. control (NS-NS-HTV); *p < 0.05 compared to LPS alone.