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Fig. 4 | BMC Anesthesiology

Fig. 4

From: Dexmedetomidine attenuates oxygen-glucose deprivation/ reperfusion-induced inflammation through the miR-17-5p/ TLR4/ NF-κB axis

Fig. 4

TLR4 overexpression and miR-17-5p silencing inhibit the anti-inflammatory and anti-apoptotic effects of DEX in PC12 cells following OGD/R. A PC12 cells were transfected with pcDNA3.1-TLR4 and miR-17-5p inhibitor, and TNF-α, IL-6, and IL-1β levels were measured by ELISA. B Apoptosis rate was detected by flow cytometry in H2O2-treated PC12 cells after transfection with pcDNA3.1-TLR4 and miR-17-5p inhibitor. C Relative expression of Bcl-2, Bax, and caspase 3 in PC12 cells transfected with pcDNA3.1-TLR4 and miR-17-5p inhibitor detected by western blot. Data are the mean ± SD of three independent experiments. *P < 0.05 vs OGD/R; #P < 0.05 vs DEX

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