Fig. 3
miR-17-5p inhibits TLR4/ NF-κB signaling, inflammation, and apoptosis in PC12 cells following OGD/R. A TLR4 expression in treatment and control groups determined by qRT-PCR. B Relative expression of p-IκBα, IκBα, p-p65, and p65 in PC12 cells detected by western analysis after transfection with miR-17-5p mimic or miR-17-5p inhibitor. NC1, negative vector control for miR-17-5p mimic; NC2, negative vector control for miR-17-5p. C PC12 cells were transfected with miR-17-5p mimic and miR-17-5p inhibitor for 48 h, then TNF-α, IL-6, and IL-1β levels were measured by ELISA. D Apoptosis rate was detected by flow cytometry in H2O2-treated PC12 cells after transfection with miR-17-5p mimic or miR-17-5p inhibitor. E Relative expression of Bcl-2, Bax, and caspase 3 detected by western analysis after transfection with miR-17-5p mimic or miR-17-5p inhibitor. Data represent the mean ± SD of three independent experiments. *P < 0.05 vs control; #P < 0.05 vs OGD/R + vehicle