Fig. 5

Ropivacaine inhibits the proliferation and migration of HaCaT cells by suppressing the PI3K/AKT/mTOR pathway. A Effect of ropivacaine on proteins expression related to PI3K/AKT/mTOR signaling pathways after IGF-1 intervention as determined by western blot analysis; blots were cut around specified molecular weights prior to separate probing with specific antibodies. Unedited blots are shown in Supplementary File. B Quantification of the western blot analysis in A; Typical images of wound scratch C and transwell assays E of HaCaT cells in response to ropivacaine after IGF-1 intervention; The statistical results of wound scratch D and transwell assays F. Error bars represent the mean ± S.D.; **P < 0.01 vs. ropivacaine- IGF- group, #P < 0.05, ##P < 0.01 vs. ropivacaine + IGF- group