Removal of EpCAM-positive tumor cells from blood collected during major oncological surgery using the Catuvab device- a pilot study

Table 1 Results of Measurement of EpCAM-Positive Tumor Cells

Subject number	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16
Cancer	Esoph.	CCA	Rectal	Esoph.	CCA	Pancr.	Gist	Colon	CCA	Coecum +Ovar.	Pancr.	Pancr.	Bile duct	Rectal	Bile duct	pCCC
Surgery blood (mL) Dilution fluid (mL)	1400 550	500 0	2800 2500	1600 1000	1100 500	1600 500	800 300	950 300	1000 700	2200 200 + 800	800 150	1700 1400	1661 900	2600 50	1400 100	800 200
Total number of tumor cells in IBS Reservoir^a	7600	0	263,076	0	14,322	108,984	108	0	0	101,525	15,529	0	0	0	0	0
Total number of tumor cells in EC^b	0	192	6835	0	2756	0	0	0	0	4163	1886	69	0	0	419	0
Total number of tumor cells in EC after filtration (LDF)^c	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

The numbers of tumor cell in the reservoir and ECs were assessed in test samples (20 ml) and calculated to the total volume. In the EC following filtration, the total volume was consumed for tumor cell assessment
CCA: cholangiocarcinoma; EC: erythrocyte concentrate; EpCAM: epithelial cell adhesion molecule; Esoph.: esophageal cancer; LDF: leukocyte depletion filter; Ovar.: ovarian cancer; Pancr.: pancreatic cancer; pCCC: perihilar cholangiocellular carcinoma
^a IBS Reservoir: Blood and purge solvent accumulated during surgery was collected in a reservoir containing a bone splinter filter (IBS/ Reservoir sample)
^b EC: Blood and purge solvent mix collected in the reservoir was centrifuged and washed using a IBS machine, resulting in an EC (EC sample)
^c LDF: EC was filtered using a 40 μM LDF (Haemonetics), resulting in an EC containing almost no white blood cells (LDF sample)

ISSN: 1471-2253