Table 2 Primer sequences for PCR
PCR Assay | Oligonucleotide Sequence (5 ‘-3 ‘) | Gene bank number |
|---|---|---|
PPIA | fw-CTTTCACAgAATAATTCCAggATT | NM_214353 |
rev-ggACAAgATgCCAggACC | ||
fl-ATgCTTCAggATAAAATTCTCATCATCAAA | ||
cy5-TTCTCTCCATAgATggACTTgCCACCA | ||
IL 6 | fw-CCAATCTgggTTCAATCAggA | NM_214399 |
rev-gTggTggCTTTgTCTggATTC | ||
fl-TgTCgAggCTgTgCAgATTAgTACCA | ||
cy5-gCACTgATCCAgACCCTgAggCAA | ||
TNFalpha | fw-CCCAgAAggAAgAgTTTCCA | NM_214022 |
rev-CggCTTTgACATTggCTACA | ||
fl-ggCCCAAggACTCAgATCATCgTC | ||
cy5-CAAACCTCAgATAAgCCCgTCgC | ||
iNOS | fw-gATggCACCATCATAggggAC | NM_001143690 |
rev-ggCACCCTgggAACTCAA | ||
fl-TGGAACACCCCAAATACGAGTGGTTCC | ||
cy5-GGAGCTGGAGCTGAAGTGGTACGCCC | ||
IL8 | fw-CAAgAgTAAgTgCAgAACTTCgAT | NM_213867 |
rev-CAggCAgACCTCTTTTCCAT | ||
fl-CACCTTTCCACCCCAAATTTATCAAg | ||
cy5-AACTgAgAgTgATTgAgAgTggACCCC |