Fig. 1
From: The expression of kappa-opioid receptor promotes the migration of breast cancer cells in vitro
Expression of OPRK1 in breast cancer cells and normal human mammary epithelial cells. A OPRK1 protein expression was determined by western blot in MCF-10A, MDA-MB-213, MDA-MB-435 and MCF-7 cells. GAPDH was used as loading control. B OPRK1 mRNA expression was determined by RT-qPCR in MCF-10A, MDA-MB-213, MDA-MB-435 and MCF-7 cells. C-D MDA-MB-231, MCF-7 and MCF-10A cells were transfected with OPRK1 siRNA and the OPRK1 protein expression was determined by western blot. GAPDH was used as loading control. E OPRK1 mRNA expression was determined by RT-qPCR in MDA-MB-231, MCF-7 and MCF-10A cells transfected with OPRK1 siRNA